The Hit List

Marks enemies at random on the screen. Killing a marked enemy will result in a permanent damage increase of 0.5 to a cap of 20 damage. This means that the effect of this item stops adding permanent bonus damage after killing 40 marked enemies. Enemies are marked at random and if they are not killed quickly enough another enemy will be marked instead. The number of killed enemies for this item can be seen above the abilities bar on the HUD, indicated by tally marks.

Unlocking
To unlock this item, the Bandit's fourth ability, /, has to consecutively kill an enemy 15 times (although (/ does not have to be used consecutively). The easiest way to do this is to weaken enemies with other abilities and then use Lights Out when they are weak enough to die in one hit.
 * It is highly recommended to use the as using  a lot when on the first level will instantly kill most enemies.

Character Synergies
It synergies with a lot of high damaging survivors like sniper, huntress, bandit, etc.

Item Synergies
It synergies with a lot of AoE (area of effect) damage Like gasoline and will o the wisp you need to pile them up before you knock them down usually in a large group there will be 1-2 marked enemies (i know only 1 can be marked but if one dies there is a medium chance of getting another mark there instantly)

Bugs
Sometimes it is possible for to mark a Magma Worm that's still above ground but already dead. In this case, you are unable to complete the mark.